Thomas Cech
Many nuclear DNA-binding proteins also bind RNA, often inexplicably. For example, the histone methyltransferase Polycomb Repressive Complex 2 (PRC2) binds nuclear RNAs with high affinity and limited specificity, but it has been highly controversial whether these interactions are functionally important. We have used CRISPR-Cas9 genome editing, live cell single molecule imaging, and genome-wide and transcriptome-wide experiments to reveal the importance and the mechanism by which RNA binding regulates PRC2 recruitment to target genes.
Abstracts this author is presenting:
