Although the inheritance of genetic information has long been established, in recent years, it has become apparent that epigenetic marks can also be transmitted from one generation to the next. This phenomenon, where gene expression patterns are transmitted from one generation to the next with no change to the DNA sequence, is referred to as transgenerational epigenetic inheritance (TEI). It has been observed in a range of species, including Mus musculus, Drosophila melanogaster, and Caenorhabditis elegans and has implications for natural selection, viral defence, and germline immortality. The research presented here utilizes C. elegans in an RNA interference (RNAi) assay to assess the inheritance of gene silencing, involving silencing of a gfp transgene by exposure of parent animals to an RNAi trigger and assessing following generations for inheritance of this silencing in the absence of this trigger. Previous work has distinguished three distinct parts within TEI, including initiation of gene silencing, the establishment of silencing inheritance within the parental germline, and the maintenance of this silencing signal within the progeny. A number of genes have been implicated as involved in one or more of these processes, including histone methyltransferases and Argonaute proteins. This study examined the RNA helicase emb-4, the GHKL ATPase morc-1, and the predicted histone methyltransferases set-9 and set-26. The results of the study implicate emb-4 and set-9 as involved in the establishment of a heritable silencing signal and morc-1 and set-26 as involved in the maintenance of this signal over successive generations. Further research was conducted to determine whether emb-4 and set-9 are required within the parental generation in order to transmit a heritable silencing signal or within the first generation of progeny to receive such a signal. Our results revealed that both emb-4 and set-9 are required within the parent animals for proper TEI.