The metabolism of tRNA is becoming an increasingly complex field of research. tRNA are subject to diverse epigenetic modifications, their abundance can be adapted to the translational needs of the cell and additional regulatory roles are attributed to fragments generated during decay. These processes hold capacity to significantly diversify tRNA function from the traditional role as stable translational machinery components. Our research probes the idea that derailment of normal co-transcriptional RNA processing is a universal response to stress in S. cerevisiae. It is known that splicing and alternative polyadenylation are regulated during stress. Here, we describe a new aspect of tRNA regulation which rapidly responds to environmental stress. We used an in-house whole transcriptome RNA-seq method to capture early changes to gene expression and poly(A) tail length distribution during cellular adaptation to osmotic stress. In addition to changes in mRNA expression and polyadenylation-state, we identified rapid polyadenylation of rRNA and pre-tRNAs within 5 minutes. Unprocessed, short poly(A) tailed, pre-tRNA transcripts accumulated transiently, being removed from the transcriptome following adenylation. The non-canonical poly(A) polymerase Trf4p is required for this polyadenylation, suggesting tRNA adenylation is carried out by the Nuclear TRAMP complex. Interestingly, the process appears to primarily target proline and glutamine accepting tRNAs, suggesting a selective mechanism. Proline and glutamine were previously described as osmoprotectants in bacteria, and mRNA encoding the proline transporter PUT4 is upregulated later in the stress response. We propose the pre-tRNA polyadenylation, and the uncoupling of normal processing of these tRNA toward a decay pathway represents a previously uncharacterised arm of cell biology that allows yeast to rapidly adjust their intracellular environment to adapt to osmotic stress preceding adaptation through the proteome.